{"id":185,"date":"2014-02-25T11:42:28","date_gmt":"2014-02-25T01:42:28","guid":{"rendered":"http:\/\/paulyeatman.net.au\/?p=185"},"modified":"2016-08-23T16:41:09","modified_gmt":"2016-08-23T06:41:09","slug":"dehydrated-culture-media-used-in-media-fill","status":"publish","type":"post","link":"https:\/\/paulyeatman.net.au\/index.php\/2014\/02\/25\/dehydrated-culture-media-used-in-media-fill\/","title":{"rendered":"Dehydrated Culture Media used in &#8220;Media Fill&#8221;"},"content":{"rendered":"<p><strong>The Question posed on <a href=\"http:\/\/www.linkedin.com\/groups\/89686\/89686-5839126357237329922\" target=\"_blank\">LinkedIn<\/a>.<\/strong><\/p>\n<p>&#8220;Dehydrated Culture Media used in &#8220;Media Fill&#8221;. Does the powder specification need to be sterile (irradiated) or it can be sterilized by filtration (0.22\u00b5m) just after browth preparation in tank?&#8221;<\/p>\n<p><strong>My advice:<\/strong><\/p>\n<p>Your starting dehydrated media should meet your existing limits for raw materials with regards to microbial load.<\/p>\n<p>If your standard procedure is to formulate your product in an ancillary room and sterile filter it on the way to the filling heads, then I would include this in your sterile media fill trials as it simulates your normal batch process.<\/p>\n<p>A batch simulation with no interventions should demonstrate your filtration sterilises your &#8220;product&#8221;. Once that&#8217;s done, you can then incorporate interventions into your sterile media fill trials.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Question posed on LinkedIn. &#8220;Dehydrated Culture Media used in &#8220;Media Fill&#8221;. Does the powder specification need to be sterile (irradiated) or it can be sterilized by filtration (0.22\u00b5m) just after browth preparation in tank?&#8221; My advice: Your starting dehydrated media should meet your existing limits for raw materials with regards to microbial load. If [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[21],"tags":[23,28],"class_list":["post-185","post","type-post","status-publish","format-standard","hentry","category-advice-provided","tag-growth-media","tag-process-simulation"],"_links":{"self":[{"href":"https:\/\/paulyeatman.net.au\/index.php\/wp-json\/wp\/v2\/posts\/185","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/paulyeatman.net.au\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/paulyeatman.net.au\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/paulyeatman.net.au\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/paulyeatman.net.au\/index.php\/wp-json\/wp\/v2\/comments?post=185"}],"version-history":[{"count":3,"href":"https:\/\/paulyeatman.net.au\/index.php\/wp-json\/wp\/v2\/posts\/185\/revisions"}],"predecessor-version":[{"id":439,"href":"https:\/\/paulyeatman.net.au\/index.php\/wp-json\/wp\/v2\/posts\/185\/revisions\/439"}],"wp:attachment":[{"href":"https:\/\/paulyeatman.net.au\/index.php\/wp-json\/wp\/v2\/media?parent=185"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/paulyeatman.net.au\/index.php\/wp-json\/wp\/v2\/categories?post=185"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/paulyeatman.net.au\/index.php\/wp-json\/wp\/v2\/tags?post=185"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}